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Abstract Detail

Physiological Section

Tyack, Nicholas B. [1], Krosnick, Shawn E. [1].

Optimization of micropropagation techniques for ex-situ conservation of the phylogenetically significant species Passiflora lancetillensis.

Passiflora lancetillensis is one of four species recognized in Passiflora subgenus Decaloba section Pterosperma. While P. lancetillensis has been documented in both Honduras and Belize, the species is restricted to small patches of primary forest in nature reserves. This rarity is reflected in the small number of herbarium specimens available (< 20 worldwide), and has led to its classification as rare according to the 1998 IUCN Red List. Passiflora lancetillensis exhibits several plesiomorphic characteristics that seem to contradict its more derived position in Passiflora subgenus Decaloba. Thus, this species is of particular interest from an evolutionary standpoint, making observation of fertile living material essential. Passiflora lancetillensis does not reproduce well via traditional methods of propagation (stem cuttings, seed). Thus, to ensure the long-term survival of this species, methods for micropropagation were optimized. Optimal concentration and exposure length were determined for ethanol (70%, 60 sec) and bleach sterilization (1% sodium hypochlorite, 10 min) of leaf, stem, tendril, and axillary bud tissue. This regimen was then applied to a larger sample (five samples/plate, five plates/tissue type). Tissue was grown on MS media supplemented with IAA (11 µM) and BAP (4.5 µM) to promote the development of callus, axillary bud growth, and/or rooting, depending on the tissue type. Axillary buds exhibited the fastest growth and greatest percentage of healthy explants; the tendril, stem and leaf tissue developed callus with eventual shoot differentiation, but took much longer. Based on these preliminary results, it appears that axillary buds present the best opportunity for successful in-vitro propagation of Passiflora lancetillensis. The optimization of a high-yield procedure for propagating rare Passiflora species will facilitate both ex-situ and in-situ conservation by allowing for broad distribution of cloned explants.

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1 - Rancho Santa Ana Botanic Garden, Department of Research, 1500 North College Avenue, Claremont, California, 91711, USA

tissue culture.

Presentation Type: Poster:Posters for BSA Sections
Session: P1
Location: Event Tent/Cliff Lodge
Date: Monday, July 27th, 2009
Time: 5:30 PM
Number: P1PS001
Abstract ID:93