MSA - Ecology/Pathology
Cating, R. A. , Palmateer, A. J. , Hoy, M. A. .
Culture methods for growth and sporulation of Pseudocercospora dendrobii, P. odontoglossi, and a Pseudocercospora isolate from Bulbophyllum orchids.
Some cercosporoid fungi are notorious for slow growth and reduced conidial production when grown on artificial media. While some species may grow and sporulate readily in culture, others require special media, light, or temperature for growth and spore production. In this study, single-spore isolates of P. dendrobii, P. odontoglossi, and an undescribed Pseudocercospora isolate from Bulbophyllum orchids taken directly from diseased orchids were grown on V-8 medium at 15, 20, 25, and 30°C and under 12L/12D or 8L/16D photoperiods. Culture diameter was measured every two days and the cultures were examined for spores after 10 days of growth or, in some cases, every 24 h. The successful growth and sporulation in axenic culture of these orchid pathogens will allow pathogenicity and host-range studies, morphological studies, and phylogenetic analysis based on molecular data.
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1 - Tropical Research and Education Center, University of Florida, Plant Pathology, 18905 SW 280 St., Homestead, FL, 33031, United States
2 - University of Florida, Entomology and Nematology, 970 Natural Area Drive, Gainesville, FL, 332611, United States
Presentation Type: Poster:Posters for Topics
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM