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Abstract Detail


Economic Botany Section

Jahan, Nusrat [1], Zaidi, Mudassir [2].

Development of an efficient protocol for the micro propagation of Carnation using Plant Tissue Techniques.

A simple and efficient micro propagation protocol was developed for Dianthus caryophillus a beautiful ornamental plant using shoot tips and nodal segments as explants. MS, WPM, N6 media. The explants were cultured in MS medium supplemented with BAP and kinetin incubated for 6 weeks and subculture in MS medium on to fresh medium after every 2 weeks. The proliferated culture was cultured in MS with 3% sucrose for elongation of shoots, formation of roots and futher proliferation of axillary shoots separation. Large numbers of shoots were produced in medium containing 3.50mg/L BAP and 1mg/l Kinetin. Rooting started on the same media when plant attained height of 3cm. After the production of roots the plants were transplanted individually in small pots and kept in the close tunnel where the humidity was approximately 70 %. After acclimatization plants were shifted to green house. The exponential rise in plants was very encouraging for the development of an efficient micropropagation system.


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1 - Balochistan Unversity of IT, Engineering& Management Sciences, Biotechnology & Informatics, Plant Tissue Culture Lab, BUITEMS, Quetta, Quetta, Balochistan, 83700, Pakistan
2 - University of Balochistan, Botany, Sariab road, Quetta, Balochistan, 87300, Pakistan

Keywords:
tissue culture
Carnation.

Presentation Type: Oral Paper:Papers for BSA Sections
Session: 48
Location: Magpie B/Cliff Lodge - Level B
Date: Tuesday, July 28th, 2009
Time: 1:30 PM
Number: 48001
Abstract ID:916


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