Nepal, Madhav P. , Fehlberg, Shannon D. , Ferguson, Carolyn J. .
Developing and applying low-copy nuclear regions for phylogenetic study in the genus Phlox (Polemoniaceae).
Phlox, the largest genus of the well-studied family Polemoniaceae, presents intriguing questions of diversification relative to ecology, hybridization and polyploidy. We are particularly interested in understanding relationships of a set of taxa in the desert southwest, part of the P. longifolia species complex. While ITS and cpDNA data provide some phylogenetic information for the group, data from additional, highly variable sequence regions are required for resolution of relationships within the species complex. We have employed a variety of approaches to identifying informative low copy nuclear regions, including the use of EST data from another member of the Polemoniaceae to identify and amplify potentially variable intron-containing regions. Approaches, challenges and successes of marker development are discussed, along with comparison of phylogenetic findings. This study benefits from an understanding of cytotype variation in the group based on flow cytometry data: inter-population variation in ploidy level occurs within recognized species in some cases, and undetected polyploidy has hampered some previous phylogenetic work. We briefly discuss related population genetic work in the species complex and place this study within the context of ongoing phylogenetic investigation of the genus as a whole.
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1 - Kansas State University, Herbarium and Division of Biology, Ackert Hall, Manhattan, Kansas, 66506-4901, USA
2 - Desert Botanical Garden, Research, 1201 N. Galvin Pkwy., Phoenix, AZ, 85008, USA
low copy nuclear markers
Presentation Type: Poster:Posters for BSA Sections
Location: Event Tent/Cliff Lodge
Date: Monday, July 27th, 2009
Time: 5:30 PM