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Abstract Detail


Population Genetics

Aldrich-Wolfe, Laura [1], Nelson, Berlin D [1].

Genetic variation of the plant pathogen Sclerotinia sclerotiorum on four crop host species in the north central United States.

Sclerotinia sclerotiorum is an important pathogen of many of the most commonly-grown crops in the north central United States, yet little is known about how this pathogen varies either genetically or in virulence across the region and across the different crop host species. In fall 2008, we obtained 154 isolates of S. sclerotiorum from four crops (canola, dry bean, soybean, and sunflower) in ten north central states (North Dakota, South Dakota, Nebraska, Minnesota, Iowa, Wisconsin, Michigan, Indiana, Ohio and Illinois) and surrounding areas. Of these isolates, 144 have been successfully subcultured and stored for mycelial compatibility group, microsatellite, and virulence analyses. As a first step in assessing genetic variation of S. sclerotiorum in the region, we are conducting mycelial compatibility group analysis. We have currently screened 98 isolates from canola, dry bean, soybean, and sunflower in the Red River Valley of North Dakota and Minnesota. These isolates form fewer than 43 mycelial compatibility groups, which vary in size from 1-16 isolates. Seven of these mycelial compatibility groups each contain at least five isolates, while 26 mycelial compatibility groups contain only one isolate. To date, mycelial compatibility groups appear to be shared across geographic regions and host crops. Local areas may contain as much genetic diversity as broad geographic regions. Microsatellite analysis will clarify the extent to which populations of S. sclerotiorum vary genetically among host crop species and across the Midwest.


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Related Links:
National Sclerotinia Initiative


1 - North Dakota State University, Plant Pathology, Dept 7660, Box 6050, Fargo, ND, 58108-6050, USA

Keywords:
Sclerotinia sclerotiorum
Genetic diversity
multiple hosts
geographic variation
gene flow
Soybean
Canola
dry bean
Sunflower
mycelial compatibility group
microsatellites.

Presentation Type: Oral Paper:Papers for Topics
Session: 40
Location: Cottonwood D/Snowbird Center
Date: Tuesday, July 28th, 2009
Time: 11:45 AM
Number: 40010
Abstract ID:721


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