MSA - Cell and molecular biology/Physiology & Genetics
Barnes, Irene , Kirisits, Thomas , Wingfield, Michael J. , Wingfield, Brenda D .
Diagnostic markers reveal two species of Dothistroma in Hungary.
Two different fungal pathogens having similar morphology cause Dothistroma needle blight (DNB) of pines. These are Dothistroma pini which occurs in the U.S.A., Russia and Ukraine and D. septosporum which occurs widely in the Northern Hemisphere and has been introduced into Southern Hemisphere pine plantations. During a population study of D. septosporum isolates from Hungary, microsatellite markers indicated the possible presence of a second species of Dothistroma. The aim of this investigation was to identify the causal agent of DNB occurring on Pinus nigra in Hungary. Samples were collected at two areas in the Highlands of Lake Balaton. Single conidial isolations were made from single conidiomata on a needle, several conidiomata on the same needle and conidiomata from different trees. DNA extracted from these cultures and directly from conidiomata on needles was screened with a combination of four different markers. These included Dothistroma species-specific mating type primers, sequencing the rDNA ITS region, a species-specific ITS-RFLP and a diagnostic microsatellite marker (Doth_A). The species-specific ITS-RFLP profiles and the Doth_A marker indicated that D. septosporum and D. pini were present at both collection sites in Hungary. These results were confirmed with the rDNA ITS sequence data. The analyses further showed that both DNB pathogens can occur on the same tree, the same needle and even in a single conidioma. The mating type primers revealed the presence of both MAT1 and MAT2 for D. septosporum but only the MAT2 for D. pini. We report, for the first time, the occurrence of both DNB pathogens in the same area. Furthermore, D. pini is recorded for the first time in Hungary and Central Europe. The molecular markers applied in this study were sufficiently robust to identify and differentiate between the two DNB pathogens, both in culture and using DNA extracted directly from conidiomata on needles.
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1 - Forestry and Agricultural Biotechnology Institute (FABI), Department of Genetics, University of Pretoria, Pretoria, 0002, South Africa
2 - Institute of Forest Entomology, Forest Pathology and Forest Protection, Department of Forest and Soil Sciences (DFS), University of Natural Resources and Applied Life Sciences, Vienna (BOKU), Vienna, Austria
3 - Forestry and Agricultural Biotechnology Institute (FABI), Department of Genetics,, University of Pretoria, Pretoria, 0002, South Africa
4 - Forestry and Agricultural Biotechnology Institute (FABI), Department of Genetics, University of Pretoria, Pretoria, 0002, South Afica
Presentation Type: Poster:Posters for Topics
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM