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Abstract Detail


MSA - Cell and molecular biology/Physiology & Genetics

Hallen-Adams, Heather E [1], Cavinder, Brad L [2], Trail, Frances [3].

Calcium channels MID1 and CCH1 are required for ascospore discharge in Gibberella zeae.

The majority of ascomycetes forcibly discharge their ascospores, and yet the mechanism of discharge is not well understood. We are studying two calcium channels, MID1 and CCH1, involved in ascospore discharge in the filamentous fungus Gibberella zeae (anamorph Fusarium graminearum). In G. zeae, cch1Δ mutants were observed to have slower vegetative growth rates, abnormal sexual and ascospore development, and reduced ascospore discharge as compared to wild type (Hallen and Trail 2008). The mid1Δ mutants grew slightly slower, discharged ascospores at a slightly higher level, and developed abnormal ascospores with much greater frequency than the cch1Δ mutants. Complementation of the mid1Δ mutants with wild type MID1 sequence restored wild type characteristics. mid1Δcch1Δ double mutants were also characterized and compared to the characteristics of the single mutants. Complete functional analysis of both genes and their effects on ascus function is presented.


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1 - Michigan State University, Department of Plant Biology & DOE Plant Research Laboratory, 166 Plant Biology Laboratories, East Lansing, MI, 48824-1312, USA
2 - Michigan State University, Genetics Graduate Program, 166 Plant Biology Laboratories, East Lansing, MI, 48824-1312, USA
3 - Michigan State University, Department of Plant Biology & Department of Plant Pathology, 166 Plant Biology Laboratories, East Lansing, MI, 48824-1312, USA

Keywords:
Fusarium graminearum
ballistospore discharge
Calcium.

Presentation Type: Poster:Posters for Topics
Session: P2
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM
Number: P2CG016
Abstract ID:620


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