MSA - Systematics/Evolution
Wolfe, Benjamin E. , Pringle, Anne .
The saprotrophic-biotrophic continuum in the Pluteoid clade: a ripe opportunity for comparative genomics of the ectomycorrhizal symbiosis.
Recent whole genome sequence data for ectomycorrhizal (EM) fungi has greatly advanced our understanding of the molecular genetics of the ectomycorrhizal symbiosis. However, we still lack a detailed understanding of the differences in genomic architectures between ectomycorrhizal and saprotrophic fungi. In this poster, we propose that the Pluteoid clade (Pluteaceae, Amanitaceae, Pleurotaceae and Limnoperdonaceae) in the Agaricales is an ideal system for the comparative genomics of the ectomycorrhizal symbiosis. Phylogenetic reconstructions strongly support a monophyletic clade of EM Amanita species, suggesting a single origin of the EM symbiosis. Stable isotopes of carbon, culture growth assays, and EM root tip samples from the field suggest that there is a gradient in saprotrophic capabilities, even within the EM Amanita clade. Using suppression subtractive hybridization with cultures of several saprotrophic Pluteoid taxa (Amanita thiersii, Amanita manicata, Volvariella volvacea, Pluteus cervinus) grown on cellulose and glucose, we are identifying genes involved in cellulose degradation. We will assess the presence and activity of these genes across the Pluteoid phylogeny, including in EM Amanita species, to further characterize the saprotrophic-biotrophic continuum in this group. Whole genome sequencing projects of selected Pluteoid species in conjunction with currently available genomic resources for Amanita species would provide a powerful system for comparative genomics of the evolution of the ectomycorrhizal symbiosis.
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Benjamin Wolfe-Pringle Lab Website
1 - Harvard University, Department of Organismic and Evolutionary Biology, 16 Divinity Ave, Cambridge, MA, 02138, USA
Presentation Type: Poster:Posters for Topics
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM