MSA - Cell and molecular biology/Physiology & Genetics
Wang, Zheng , Kin, Koryu , Lopez-Giraldez, Francesc , Clark, Travis , Townsend, Jeffrey .
Compare genetic behavior between mating types of Neurospora crassa during vegetative growth using a whole genome transcription profiling with multi-targeted priming (MTP).
Neurospora crassa is a heterothallic fungus, whose sexual development is triggered right after the crossing of strains from both mating types, MAT-A and MAT-a. Vegetative incompatibility between these two mating types has been observed in Neurospora crassa for a long time, but the mechanism is not known. To investigate the relative gene expression levels and changes in gene expression between the two mating types during the vegetative growth (asexual stage), total RNA and mRNA were sampled from both mating types at three time points, 36hr, 60hr, 96hr, corresponding to the early, middle, and late development stages. Combined multi-targeted priming (MTP) and Oligo(dT) priming was applied to these samples in a reverse transcription step to recover expressed genes into the cDNA pools, and a circuit design of hybridization among these cDNA samples was analyzed using BAGEL, a Bayesian analysis of gene expression level. Our preliminary results of microarray profiling suggested gene expression level changes throughout the vegetative development in and between both mating types, and most significant changes between the mating types were observed during the late development of the asexual colonies.
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1 - Yale University, Department of Ecology and Evolutionary Biology, Osborne Memorial Labs 226, 165 Prospect Street, New Haven, CT, 06520, USA
Presentation Type: Poster:Posters for Topics
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM