Bruederle, Leo , Bull, Roger D. , Ginter, Anna , MacPhail, Alex , Starr, Julian R. .
Cross-species Amplification for 36 Microsatellite Loci in Tribe Cariceae (Cyperaceae) and Their Potential for Systematic Research on Sedges.
Cross-species amplification was examined for 36 previously published microsatellite primer pairs in 15 species representing the phylogenetic breadth of tribe Cariceae (Cyperaceae). Most primer pairs cross-amplified loci for at least two species, while 28 exhibited the potential to amplify loci within or even across major tribal clades. Following PCR optimization, 20 primer pairs were selected to further determine their utility for systematic research in Carex section Ceratocystis. Data were collected for all North American species comprising this section in order to examine species limits and document hybridization, as well as to describe genetic diversity and structure within Carex lutea, a federally protected sedge from eastern North America. Of these microsatellite loci, ten were found to be taxonomically informative, with species-specific alleles. As such, it was possible to document hybridization using these primer pairs. In addition, five loci were polymorphic for C. lutea, with 2-5 alleles per locus. These loci suggest that genetic diversity within this species is significantly higher than that reported using allozymes, with strong patterns of genetic structure within and among populations. This research reveals that previously published microsatellite primer pairs may have broad systematic application within tribe Cariceae.
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1 - University of Colorado Denver, Department of Biology, Campus Box 171, PO Box 173364, Denver, CO, 80217-3364, USA
2 - Canadian Musuem of Nature, Research Division, P.O. Box 3443, Station D, Ottawa, ON, K1P 6P4, Canada
3 - University of Ottawa, Department of Biology, PO Box 450, Station A, Ottawa, ON, K1N 6N5, Canada
Presentation Type: Oral Paper:Papers for BSA Sections
Location: Cottonwood D/Snowbird Center
Date: Wednesday, July 29th, 2009
Time: 2:00 PM