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Abstract Detail


MSA - Systematics/Evolution

Setaro, Sabrina [1], Wei, Michael [2], Garnica, Sigisfredo [3], Kron, Kathleen A. [4], Gker, Markus [5].

High molecular diversity of Sebacinales associated with Neotropical Ericaceae, as revealed by a novel clustering optimization technique.

Sebacinales are fungi which colonize fine roots of Ericaceae to form mycorrhizas for interchange of nutrients. Direct sequencing of ribosomal DNA of Sebacinales from mycorrhizas revealed a high diversity of sebacinalean sequences. However, the number of species could not be determined because the sequences did not match described Sebacinales species. A traditional description of Ecuadorian Sebacinales is not possible as fruiting bodies are lacking and no culture isolates exist. However, knowledge of species delimitation in Ecuadorian Sebacinales is important for ecological and applied studies of Sebacinales as well as their hosts, the Neotropical Ericaceae. One aim of our study was to develop a concept to delimitate Sebacinales on the molecular level using sequence information of the ribosomal DNA. We defined molecular operational taxonomic units (MOTUs) for Sebacinales obtained by a novel clustering optimization technique and inferred Sebacinales B phylogeny using a Maximum Likelihood as well as a Maximum Parsimony approach. The cluster optimization technique was revealed to be a suitable tool to deal with molecular diversity of Sebacinales B and could be applied to other organismic groups with similar problems in the future.


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1 - Wake Forest University, Department of Biology, 113 Winston Hall, PO Box 7325 Reynolda Station, Winston-Salem, North Carolina, 27109, USA
2 - Universitaet Tuebingen, Spezielle Botanik und Mykologie, Auf der Morgenstelle 1, Tuebingen, 72076, Germany
3 - Universitaet Tuebingen, Spezielle Botanik und Mykology, Auf der Morgenstelle 1, Tuebingen, 72076, Germany
4 - Wake Forest University, Department of Biology, PO Box 7325, 226 Winston Hall, Winston-Salem, North Carolina, 27109-7325, USA
5 - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhofferstr. 7b, Braunschweig, 38124, Germany

Keywords:
Sebacinales
molecular diversity
molecular phylogenetics.

Presentation Type: Poster:Posters for Topics
Session: P2
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM
Number: P2SE036
Abstract ID:526


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