| Abstract Detail
MSA - Cell and molecular biology/Physiology & Genetics van der Nest, Magriet A [1], Slippers, Bernard [1], van Zyl, Karlien [1], Stenlid, Jan [2], Wingfield, Michael J [3], Wingfield, Brenda D [4]. Gene expression during vegetative incompatibility in Amylostereum areolatum. Typical of filamentous fungi, the hyphae of genetically similar Amylostereum areolatum individuals sharing the same allele specificities at their heterokaryon incompatibility (het) loci are able to fuse. Interacting hyphae of genetically dissimilar individuals with different allelic specificities at their het loci undergo cell death, thus preventing hyphal fusion from persisting. Even though cell death associated with vegetative incompatibility represents the best studied form of programmed cell death (PCD) in fungi, the mechanism of cell death by incompatibility is unclear. To better understand PCD by incompatibility in A. areolatum, suppression subtractive hybridization (SSH) followed by pyrosequencing was used to identify selectively expressed genes upregulated during vegetative incompatibility in this fungus. Real-time RT-PCR was employed to verify the SSH results. The transcripts identified using this method share homology with genes associated with various cellular processes, including cell death, adhesion, transport, stress responses and metabolism. These results contribute to the identification of the underlying processes and pathways involved in vegetative incompatibility in A. areolatum and made it possible to reconstruct the likely chain of events occurring during PCD in this and other fungi.
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1 - Forestry and Agricultural Biotechnology Institute (FABI), Department of Genetics, Fabi, University of Pretoria, Pretoria, 0002, South Afica
2 - Swedish University of Agricultural Sciences, Department of Forest Mycology and Pathology, P.O. Box 7026, SE-750 07, Uppsala, Sweden
3 - Forestry and Agricultural Biotechnology Institute (FABI), Department of Microbiology, Fabi, University of Pretoria, Pretoria, 0002, South Afica
4 - Forestry and Agricultural Biotechnology Institute (FABI), Department of Genetics, University of Pretoria, Pretoria, 0002, South Afica
Keywords:
het loci
Programmed Cell Death
Subtractive Hybridization.
Presentation Type: Poster:Posters for Topics
Session: P2
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM
Number: P2CG010
Abstract ID:501 |
