Parks, Matthew , Liston, Aaron , Cronn, RC .
Whole Chloroplast Genome Sequences Increase Infrageneric Phylogenetic Resolution: A Case Study in Pinus;.
Infrageneric plant molecular evolutionary studies document many incompletely resolved lineages, reflecting recent divergence and/or rapid radiation. At this level, the conservative substitution rate of the chloroplast genome has hindered robust phylogenetic and phylogeographic resolution. We estimated phylogenetic relationships within the genus Pinus using sequences from 39 plastomes (>4 Mbp), 37 of these generated by multiplexed massively parallel sequencing. Nearly all ingroup clades (28-30/33) resolved with ≥95% parsimony or likelihood bootstrap support. This is significantly higher than three previous Pinus chloroplast-based studies and nodal support distributions from nearly 100 published chloroplast phylogenies. Two loci (ycf1, ycf2) accounted for over half of informative sites. Complete plastome sequencing is now an efficient option for plant phylogenetic and population genetic analyses at low taxonomic levels.
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1 - Oregon State University, Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, 97331, USA
2 - Oregon State University, Department of Botany & Plant Pathology, 2082 Cordley Hall, Corvallis, Oregon, 97331-2902, USA
3 - US Forest Service, Pacific NW Research Station, Genetics, 3200 SW Jefferson Way, Corvallis, Oregon, 97331, USA
massively parallel sequencing
Presentation Type: Oral Paper:Papers for BSA Sections
Location: Wasatch B/Cliff Lodge - Level C
Date: Wednesday, July 29th, 2009
Time: 2:30 PM