Symbioses: Plant, Animal, and Microbe Interactions
Nguyen, Nhu .
Enhanced sporulation of the Metschnikowia yeast clade by Cladosporium gossypiicola.
Interactions among fungi may be common in nature, but they are not always observed in the lab. An accidental contamination of a yeast culture in a sporulation experiment revealed that the contaminating filamentous fungus, Cladosporium gossypiicola, induced sporulation of a strain of Metschnikowia. In controlled experiments, nine species within the Metschnikowia clade (M. chrysoperlae, M. corniflorae, M. noctiluminum, M. viticola, Candida chrysomelidarum, C. kofuensis, Candida picachoensis, C. pimensis, and C. sp. NRRL Y-27752) were tested by growing on dilute V8 agar with C. gossypiicola at 17ºC and observed weekly for 6 weeks. The rate of ascospore development was dramatically increased in M. corniflorae, M. noctiluminum, and M. viticola. In fact, ascospore formation was never observed after 6 weeks in two strains of M. chrysoperlae without the presence of C. gossypiicola. In addition, I observed release of ascospores from the asci, an uncommon phenomenon in the genus, by M. noctiluminum that otherwise retained the ascospores within their asci. Cladosporium gossypiicola was also able to stimulate production of sterile asci but not ascospores in two of the species, C. picachoensis and C. kofuensis. Water soluble extracts of fungal hyphae and growth medium had no effect. Physical contact with C. gossypiicola was always necessary for sporulation to occur.
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1 - University of California, Berkeley, Plant and Microbial Biology, 111 Koshland Hall, Berkeley, CA, 94720-3102, USA
Presentation Type: Poster:Posters for Topics
Location: Event Tent/Cliff Lodge
Date: Monday, July 27th, 2009
Time: 5:30 PM