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Abstract Detail


MSA - Cell and molecular biology/Physiology & Genetics

Cui, Y. [1], Money, N.P. [1].

Membrane and cytoskeletal dynamics during trap closure in the nematophagous fungus Arthrobotrys dactyloides.

Colonies of the nematode-trapping fungus Arthrobotrys dactyloides produces high densities of three-celled constricting rings within a few hours of close contact with nematodes. The closure of these traps is triggered by nematode movement into the ring lumen, causing inflation of the motor cells to three times their untriggered volume within 100 milliseconds. Here we describe the use of confocal microscopy and transmission electron microscopy to study trap initiation and the capture mechanism. Trap closure involves the rapid reorganization of cell components and we show changes in membrane conformation and cytoskeletal distribution within the ring cells. In addition, we report a new method for inducing trap formation in liquid cultures of A. dactyloides. This method will be exceedingly useful for our ongoing investigation of trap development and function using molecular biological tools.


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1 - Miami University, Botany, 700 East High Street, 316 Pearson Hall, Oxford, OH, 45056, USA

Keywords:
Nematophagous fungi.

Presentation Type: Poster:Posters for Topics
Session: P2
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM
Number: P2CG022
Abstract ID:382


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