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Abstract Detail


Economic Botany Section

Tims, Michael C.  [1], Wise, Stephen A.  [1], Sander, Lane C.  [1].

A Little Biology Goes a Long Way: Using Fungal Methods to Extract Gallic Acid, Catechins and Xanthines from Green Tea.

Green tea (Camellia sinensis), a popular beverage enjoyed world wide, has been reported to contain compounds used in treatment of cancer (Yang et al., 2008), genital warts (Gross, 2008), cardiovascular disease ((Kuriyama, 2008), and as an antimicrobial (Wu et al., 2007). Accurate measurement and detection of these compounds is necessary for assuring good manufacturing practices and in the conduct of clinical trials. As part of a collaborative effort between the National Institute of Standards and Technology (NIST) and the National Institutes of Health (NIH) Office of Dietary Supplements analytical methods have been developed for a suite of green tea containing standard reference materials (SRMs). Previous research suggested, and our experience confirmed that the metabolic pool of flavan-3-ols in green tea leaf tissue can be altered by both biotic factors and tea leaf processing. Understanding the biology and chemistry of these compounds helped guide the methodological choices. Plant primary cell walls are composed of biopolymers cellulose, hemicellulose, pectin, proteins and lignin. They also contain irregularly deposited monomeric and polymeric flavan-3-ols. Method development for measurement and certification of analytes in green tea leaf powder SRM focused on the monomeric flavan-3-ols, xanthines, gallic acid and theanine. During green tea leaf extraction, plant cell structures were disrupted and matrix components freed that interfered with recovery of flavan-3-ols. Enzyme assisted extraction increased the recovery of catechins and additional biological approaches were used to limit interference of biopolymer fragments to optimize extraction efficiency.


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1 - National Institute of Standards and Technology, Division of Analytical Chemistry, 100 Bureau Drive, MS 8392, Gaithersburg, MD, 20899-8392, USA

Keywords:
tea
Fungi
enzymes
catechin
LC-UV
LC-MS.

Presentation Type: Oral Paper:Papers for BSA Sections
Session: 48
Location: Magpie B/Cliff Lodge - Level B
Date: Tuesday, July 28th, 2009
Time: 1:45 PM
Number: 48002
Abstract ID:372


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