Liu, Xiaoyu , Reighard, Gregory , Swire-Clark, Ginger , Fan, Shenghua , Zhebentyayeva, Tetyana , Abbott, Albert , Baird, Wm. Vance .
Identification of Peach Rootstock Clones and Seedlings using Microsatellite (SSR) Markers.
Peach rootstocks are seed propagated, and seedlings are difficult to distinguish. Furthermore, once grafted no above-ground material is available for morphological identification. To avoid misidentification and to protect plant varieties and patents, DNA fingerprinting was investigated as a robust identification tool. The overall objective was to identify seedlings of the eight important peach rootstocks: Bailey, Guardian® 3-17-7, Halford, Kakamas, Lovell, Nemaguard, Nemared and S-37. Twenty-eight Simple Sequence Repeats (SSR) markers were screened on the eight parental rootstocks, and 23 exhibited polymorphism. Ten SSR markers were found to amplify as least four patterns among the eight rootstocks. Marker BPPCT008 had the most potential for identification because it amplified seven patterns and could directly identify as many as six rootstocks. Each parental rootstock was discriminated by at least one SSR marker (e.g., Lovell) or, at most, nine markers (e.g., S-37). Rootstock seedling identification was then investigated, but it was more difficult, because heterozygous patterns present in a parental rootstock/clone segregated in its seedlings (typically open-pollinated). Seedlings of several rootstocks could be identified by single SSR markers such as Nemared (BPPCT017), Bailey (CPPCT022), Kakamas (BPPCT008) and Nemaguard (BPPCT017 or EMPAS11). Seedlings of 3-17-7 and S-37 could be identified by marker combinations (e.g., EMPAS11 and pacita-16). Although Lovell and Halford seedlings were distinguished from the others by single markers (e.g., BPPCT001), there were no SSRs or marker combinations to differentiate Lovell seedlings from Halford seedlings. We conclude that the SSR markers presented in this study could be used as a practical fingerprinting system for rootstock seedling identification in peach. This technology is useful to test rootstocks for trueness to type for nursery operators and growers, and also will be helpful in protecting seed propagated proprietary rights (i.e., PVP) for breeders.
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1 - Clemson University, Horticulture Department, Poole Agr Cntr, Box 340319, Clemson, South Carolina, 29634-0319, USA
2 - Clemson University, Genetics and Biochemistry, Clemson, SC, 29634, USA
Simple Sequence Repeats
Presentation Type: Poster:Posters for BSA Sections
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM