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Abstract Detail


Genetics Section

Liu, Xiaoyu [1], Reighard, Gregory [1], Bridges, William [2], Swire-Clark, Ginger [1], Olukolu, Bode Adebowale [3], Zhebentyayeva, Tetyana [3], Abbott, Albert [3], Baird, Wm. Vance [1].

Mapping Chromosomal Regions Associated with Peach Tree Short Life Disease Syndrome.

Peach Tree Short Life (PTSL) is a complex disease syndrome involving a nematode [Mesocriconema xenoplax (Raski) Loof and de Grisse], a bacterium (Pseudomonas syringae pv. syringae van Hall), winter temperature, orchard history, and pruning. The disease occurs commonly in the southeastern U.S., resulting in large economic losses for growers. Guardian® BY520-9 rootstock was selected for its tolerance to PTSL; however, the genetic basis for this tolerance remains unknown. Nemaguard, a PTSL susceptible rootstock, and Guardian® selection 3-17-7 were crossed. Each F1 plant was selfed to create a segregating F2 population. One hundred and seventy microsatellite/simple sequence repeat (SSR) markers, each uniquely mapped to chromosomal locations on the Prunus reference genome, were used to screen the two parents and F1-11. Forty-seven SSR markers showed polymorphism between the parents and were heterozygous in F1-11. Segregation data obtained from the F2-11 population for SSR marker inheritance and PTSL-response were compiled to identify genomic regions associated with the susceptibility/tolerance to PTSL. Nine markers, distributed on 4 linkage groups, were linked with the response to PTSL through Analysis of Variance (SAS). Using Joinmap®3.0, a genetic map with seven linkage groups (217.5 cM) was constructed using 30 of the 47 polymorphic markers. Quantitative trait loci (QTLs) were detected within the upper terminus of linkage group 2 (0 to 10 cM) based on the composite interval mapping method (using PLABQTL1.2). Two SSR markers located within the QTL were found also to be associated with the response to PTSL by individual marker analysis. This region of LG-2 appears to be important because both the individual SSR analysis and the QTL analysis linked this region with the PTSL response. Developing additional SSRs or other high-resolution markers to saturate this region will further define the specific region, and ultimately identify the major candidate genes.


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1 - Clemson University, Horticulture Department, Poole Agr Cntr, Box 340319, Clemson, South Carolina, 29634-0319, USA
2 - Clemson University, Applied Economics and Statistics, 29634-0313
3 - Clemson University, Genetics and Biochemistry, Clemson, SC, 29634, USA

Keywords:
peach tree short life
Guardian rootstock
Prunus
microsatellites
Simple Sequence Repeats (SSRs)
Quantitative trait loci (QTL).

Presentation Type: Oral Paper:Papers for BSA Sections
Session: 12
Location: Cottonwood C/Snowbird Center
Date: Monday, July 27th, 2009
Time: 9:30 AM
Number: 12003
Abstract ID:332


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