Petri, Anna , Oxelman, Bengt .
Indications of horizontal gene transfer between nuclear genomes in distantly related Silene.
The extent of horizontal gene transfer, which complicates our interpretation of species phylogenies from gene trees, is poorly known for eucaryotic nuclear genomes. It has only been documented in very few cases. We have strong indications for such a case in the RNA-polymerase I encoding nuclear gene RPA2 between Silene sect. Physolychnis and members of distantly related Silene sect. Auriculate. This gene is generally present in two variants in sect. Physolychnis, and in a small number of individuals one of these copies groups with sect. Auriculate in a phylogenetic analysis. Although other factors, i.e. incomplete lineage sorting or contamination, can cause the observed patterns, these can be ruled out considering the age of the split between Physolychnis and Auriculate, and by rigorous controls in the lab. We have focused on a deeper understanding of the phylogeny of the RPA2 gene in Silene. This is achieved by direct sequencing and by using a probabilistic PCR approach to ensure that all possible variants that are present in the genome of a single Silene individual are amplified with high probability. Thorough phylogenetic analyses reveal the extent of representation of the alien RPA2 variant in Physolychnis, as well as possible time of it's introduction. The means of this introduction remains obscure.
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1 - Gothenburg University, Dpt. of Plant and Environmental Sciences, Box 461, Gothenburg, SE-40530, Sweden
2 - University of Gothenburg, Deptartment of Plant and Environmental Scineces, Box 461, Gothenburg, SE-40530, Sweden
Presentation Type: Oral Paper:Papers for BSA Sections
Location: Magpie A/Cliff Lodge - Level B
Date: Tuesday, July 28th, 2009
Time: 9:30 AM