Hawkins, Angela , Randle, Christopher P. , Archambeault, Alan D. .
Subspecific classification within Phoradendron serotinum Santalaceae): Development of microsatellite markers for assessment of population genetic structure.
Phoradendron serotinum, (leafy mistletoe) is a hemi-parasitic plant of the family Santalaceae found in the United States and Mexico. P. serotinum has been divided into four subspecies: subsp. tomentosum, subsp. macrophyllum and subsp. serotinum which occur in the eastern United States from southern New Jersey to southern Florida, through the Midwest south of Oklahoma and into Mexico, and on the west coast from Oregon to Baja and California. Subspecies angustifolium grows in isolated regions of Central Mexico. Subspecies may be difficult to identify based on morphology alone. Identification of P. serotinum subspecies in eastern Texas is especially difficult as characters that are otherwise diagnostic of subspecies do not adequately separate three of the subspecies (macrophyllum, tomentosum, and serotinum) that overlap in this region. Molecular and morphometric analyses are being utilized to resolve taxonomic confusion within Phoradendron serotinum. Total genomic DNA has been isolated from fresh tissue of more than 350 Phoradendron serotinum samples acquired from New Jersey, Delaware, Maryland, North and South Carolina, Georgia, Florida, Alabama, Mississippi, Louisiana and Texas. Microsatellite regions have been isolated using a hybridization-capture method. Primer sequences have been identified and optimized for molecular assessment of genetic differentiation among putative subspecies.
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1 - Sam Houston State University, Department of Biology, 1900 Avenue I, Huntsville, Texas, 77340, US
2 - Sam Houston State University, Department of Biological Sciences, 1900 Avenue I., Huntsville, TX, 77340
3 - Sam Houston State University, Department of Biology, 1900 Avenue I, Huntsville, Texas, 77340, USA
Presentation Type: Oral Paper:Papers for BSA Sections
Location: Cottonwood D/Snowbird Center
Date: Tuesday, July 28th, 2009
Time: 1:30 PM