MSA - Systematics/Evolution
Lefevre, Emilie , Brooks, Micheal C. , Blackwell, Will H. , Powell, Martha .
Genotypic Variation among Isolates of the Aquatic Chytrid, Rhizoclosmatium.
Rhizoclosmatium is a chytrid that is most commonly found on exuviae of insects and other sources of chitin in aquatic systems. It includes four described species, but R. globosum is the most frequently reported species. Among chytrid species, we have only limited samplings of the range in genotypes of multiple isolates of a single morphospecies from widespread geographic locations and ranging environmental habitats. Moreover, the significance of the role of chytrids in chitin degradation in freshwater has not been established. The objective of this study was to collect multiple isolates of R. globosum from the same and different habitats to determine the number of genotypes that could be detected based on ribosomal gene sequence differences. From these analyses, the degree of genotypic variation within R. globosum necessary to define the limits of the phylogenetic species was determined. Based on ribosomal gene sequence analysis of multiple isolates of R. globosum in culture, species-specific primers were developed that allow the quantification of the abundance of R. globosum in aquatic habitats using real-time PCR methods. Results of this study are vital in ascertaining the ecological role of R. globosum in chitin degradation in freshwater habitats.
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1 - The University of Alabama, Biological Sciences, Box 870344, Tuscaloosa, AL, 35487, USA
2 - University of Alabama, Department of Biological Sciences, Po Box 870344, Tuscaloosa, Alabama, 35487, USA
Presentation Type: Poster:Posters for Topics
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM