Bryological and Lichenological Section/ABLS
Hariharan, G.N. , Ramanathan, Valarmathi , Parida, Ajay , Prashanth, S.R. , Prithiviraj, Bharath .
Polyketide synthase (PKS) sequence data confirm that Roccella montagnei Bel. emend. And Roccella belangeriana D.D. Awasthi may belong to one distinct monophyletic group.
Roccella montagnei Bél. emend. Awas. & Roccella belangeriana Awasthi were keyed out as distinct species based on their reproductive strategies but the two species are similar in their morphology and secondary chemistry.The phylogenetic relationship analysis between Roccella montagnei Bél. emend. Awas. and Roccella belangeriana Awasthi using Maximum parsimony method (MEGA 3.1) based on Ketosynthase domain sequences of Polyketide synthase gene and based on the earlier study on ITS sequences of the nuclear ribosomal DNA (Prashanth et al.,2008) clustered the two species in a single clade indicating that these two species are the same. The anti-microbial potentialities of secondary compounds mentioned above against human bacterial pathogens were established using TLC bio-autographic overlay assay (Balaji et al. 2006). With this background, this study adopts amplification polyketide synthase (PKS) domain. Degenerate primers designed based on previously reported PKS sequences were used to isolate the KS domain using total genomic DNA isolated from the lichen thalli. The putative Ketosynthase domain region of 552 bp of both R. montagnei and R. belangeriana were amplified using KS region specific primers KS degenerate primers LC1-1M - 5′ GACCCGMGGTTYTTYAAYATG 3′, LC2C-1M -5′GTGCCGGTGCCRTGCATYTC 3′ followed by sequencing. The results suggest that neither R.montagnei nor R.belangeriana form monophyletic groups in any of the parsimony analyses. We conclude that the PKS data (GenBank: EF682016, EF569220, EF569219, EF569218) investigated suggests that there are no natural groups corresponding to their reproductive strategies. The parsimonious analyses and subsequent clading pattern obtained place R.montagnei and R.belangeriana species pair in a well-supported monophyletic group of intermixed specimens which revealed a lack of distinct lineages between the two species corresponding to their reproductive strategies which was as reaffirmed by Buschborm and Muller (2005).
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Balaji et al.2006
Prashanth et al.2008
1 - University of Madras, M.S.Swaminathan Research Foundation, Lichen Ecology and Bioprospecting Laboratory, 3rd cross street, Taramani Institutional Area, Taramani, Chennai, Tamil Nadu, 600113, INDIA
Presentation Type: Oral Paper:Papers for BSA Sections
Location: Superior B/Cliff Lodge - Level C
Date: Tuesday, July 28th, 2009
Time: 10:15 AM