Neubig, K. M. , Whitten, W. M. , Williams, N. H. , Moore, Michael .
Phylogenetic utility of ycf1 in Orchids part 2: bigger is better.
Plastid DNA sequences have been favored by systematists for reconstructing phylogenies of plant groups because of their variability and ease of amplification. The utility of any DNA region for phylogenetic analysis is determined by ease of amplification and alignment, and variability across broad taxon sampling. Often, a compromise must be made between using relatively highly conserved coding regions and highly variable introns or intergenic spacers. Analyses of a combination of these types of DNA regions yield phylogenetic structure at various levels of a tree (i.e., along the spine and at the tips of the branches). Here we demonstrate the phylogenetic utility of a largely unused plastid protein-coding gene ycf1 in orchids. Although recent work on phylogenetics using this gene region has focused on the ~1600 bps portion on the 3’ end only, the entire gene region, ~6000 bps total in length, shows extensive variation. All portions of ycf1 examined are highly variable yet alignable across Orchidaceae, and are phylogenetically informative at various taxonomic levels. In Orchidaceae, ycf1 is much more variable than matK both in total number of parsimony informative characters and percent variability. Although we demonstrate the phylogenetic utility of ycf1 only in orchids, it is likely to be similarly useful among other plant taxa that possess ycf1.
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1 - University of Florida, Department of Botany, 220 Bartram Hall, P.O. Box 118526, Gainesville, Florida, 32611-8526, USA
2 - University of Florida, Florida Museum of Natural History, Gainesville, Florida, 32611-7800, USA
3 - Oberlin College, Biology Department, 119 Woodland Street, Science Center K111, Oberlin, Ohio, 44074-1097, USA
Presentation Type: Poster:Posters for BSA Sections
Location: Event Tent/Cliff Lodge
Date: Monday, July 27th, 2009
Time: 5:30 PM