Unable to connect to database - 03:28:54 Unable to connect to database - 03:28:54 SQL Statement is null or not a SELECT - 03:28:54 SQL Statement is null or not a DELETE - 03:28:54 Botany & Mycology 2009 - Abstract Search
Unable to connect to database - 03:28:54 Unable to connect to database - 03:28:54 SQL Statement is null or not a SELECT - 03:28:54

Abstract Detail


Recent Topics Posters

Kuske, Cheryl  [1], Xie, Gary [2], Porras-Alfaro, Andrea [3], Dunbar, John [4], Ticknor, Lawrence [5], Vilgalys, Rytas  [6], Zak, Donald [7], Schadt , Christopher [8], Megonigal, Patrick [9], Evans , R. David  [10], Hungate, Bruce [11], Eichorst, Stephanie [12], Weber, Carolyn [13], Gallegos-Graves , La Verne  [13], Bruce, David [14].

Impacts of multi-year, elevated CO2 conditions on soil fungal communities across different terrestrial ecosystems.

The effects of atmospheric CO2 enrichment have been studied for over a decade at six large Free Air CO2 Enrichment (FACE) and Open Top Chamber (OTC) field experiments across the US. These field sites represent pine and hardwood plantations, scrubland, desert, and wetlands. Using a targeted metagenomic approach, we examined the effects of elevated CO2 on the composition of soil fungal communities within and between different ecosystems. Soil fungi mediate soil carbon cycling processes, and their physiological functions have local, regional and global impacts on carbon storage on land. A combination of fungal large rRNA subunit (LSU), rRNA ITS, and functional genes were amplified and sequenced from soil DNA using Sanger and 454 pyrosequencing approaches. Replicate plots exposed to elevated and ambient CO2 were compared to determine if elevated CO2 treatment affected the composition of soil fungi, and whether fungal communities in different ecosystems responded similarly or in different ways to elevated CO2. Our Sanger sequencing approach resulted in about 3500 double pass, high quality fungal rRNA sequences of 600 800 bp in length from each of the six sites, and we are beginning to analyze these datasets. To facilitate analysis of the data, we have developed a naïve Bayesian classifier system for fungal LSU sequences. Several thousand shorter sequences were also generated from litter and soil in ambient and elevated CO2 plots at one site using 454 pyrosequencing. Fungal community composition differed greatly among the contrasting ecosystems, and between litter and soil layers. We also observed shifts in the community in elevated CO2 plots in the 454 pyrosequencing study; this observation needs to be substantiated by replicated surveys. The soil fungal community in these ecosystems is complex, differs across ecosystems, and can be stratified by soil depth. Combining the sequence-based community composition information with process measures from the soils will help determine the overall contribution of soil fungi to ecosystem response to long-term elevated CO2.


Log in to add this item to your schedule

1 - Los Alamos National Laboratory, Los Alamos, NM, Bioscience Division, Bioscience Division, B-6, M888 Los Alamos National Laboratory, Los Alamos, NM, 87545, USA
2 - DOE Joint Genome Institute (JGI) at LANL, Genome Science (B-6), Bioscience Division, MS M888 Los Alamos National Laboratory , Los Alamos, NM, 87545, USA
3 - Western Illinois University, Biological Sciences, WIU, Waggoner Hall 372 1 University Circle , Macomb, IL, 61455, USA
4 - Los Alamos National Laboratory , Los Alamos National Laboratory ,Los Alamos, NM 87545, 87545, USA
5 - Los Alamos National Laboratory , Los Alamos, 87545, USA
6 - Duke University, Duke, NC, USA
7 - University of Michigan, School of Natural Resources, 440 Church St., Ann Arbor, 48109-1041, USA
8 - Oak Ridge National Laboratory, Oak Ridge, TN, USA
9 - Smithsonian Environmental Research Center, Edgewater, MD, USA
10 - Washington State University, Pullman, WA
11 - Northern Arizona University, Flagstaff, AZ
12 - Los Alamos National Laboratory, Los Alamos, NM
13 - Los Alamos National Laboratory, Los Alamos, NM, USA
14 - Joint Genome Institute at Los Alamos National Laboratory, Los Alamos, NM, USA

Keywords:
climate change response
Elevated CO2
soil fungal community
nrDNA ITS
large subunit rDNA
metagenomics.

Presentation Type: Recent Topics Poster
Session: P2
Location: Event Tent/Cliff Lodge
Date: Tuesday, July 28th, 2009
Time: 5:30 PM
Number: P2RT038
Abstract ID:1243


Copyright 2000-2008, Botanical Society of America. All rights